RESUMO
Efficacy of the combined therapy of experimental salmonellosis was studied on albino rats with using ampicillin, gentamicin, tetracycline and immunomodulators such as levamisole and quadevite. The influence of the combined chemoimmunotherapy on the indices of the nonspecific resistance of the animals i.e. the titers of lysozyme and beta-lysines, the complement activity and the blood serum bactericidal index was investigated. An increase in the survival of the animals exposed to the combinations of tetracycline with levamisole and quadevite by comparison with the animal survival after the monotherapy was revealed. The combinations of gentamicin with quadevite and tetracycline with the immunomodulators significantly increased the blood serum bactericidal index.
Assuntos
Adjuvantes Imunológicos/uso terapêutico , Antibacterianos/uso terapêutico , Quimioterapia Combinada/uso terapêutico , Salmonelose Animal/tratamento farmacológico , Salmonella typhimurium , Animais , Atividade Bactericida do Sangue , Lisina/análogos & derivados , Lisina/sangue , Muramidase/sangue , Ratos , Salmonelose Animal/sangueRESUMO
An attempt to correct with quadevit immunodepression due to prophylactic administration of benzylpenicillin and gentamicin for 6 days provided positive results. There was a significant increase in the quantities of Ig 19S and Ig 7S after the animal immunization with the bacterial antigen, an increase in the number of the AFCs in the spleen of the albino mice in response to administration of sheep erythrocytes and an increase in the survival rate of the animals with salmonellosis. The simultaneous use of quadevit with cefamezine and erythromycin did not lower the unfavourable influence of the antibiotics on the immune status of the animals.
Assuntos
Aminoácidos/administração & dosagem , Antibacterianos/administração & dosagem , Anticorpos Antibacterianos/biossíntese , Células Produtoras de Anticorpos/efeitos dos fármacos , Cobre/administração & dosagem , Potássio/administração & dosagem , Salmonelose Animal/prevenção & controle , Salmonella typhimurium/efeitos dos fármacos , Baço/patologia , Vitaminas/administração & dosagem , Aminoácidos/farmacologia , Animais , Antibacterianos/farmacologia , Células Produtoras de Anticorpos/imunologia , Cobre/farmacologia , Modelos Animais de Doenças , Combinação de Medicamentos , Sinergismo Farmacológico , Camundongos , Potássio/farmacologia , Coelhos , Ratos , Salmonelose Animal/imunologia , Salmonelose Animal/mortalidade , Salmonella typhimurium/imunologia , Fatores de Tempo , Vitaminas/farmacologiaAssuntos
Doenças Reumáticas/etiologia , Infecções Estreptocócicas/complicações , Streptococcus pyogenes/patogenicidade , Antígenos de Bactérias/imunologia , Proteínas de Bactérias , Tecido Conjuntivo/imunologia , Humanos , Doenças Reumáticas/microbiologia , Infecções Estreptocócicas/imunologia , Infecções Estreptocócicas/microbiologia , Streptococcus pyogenes/imunologia , Estreptolisinas/efeitos adversos , Virulência/imunologiaRESUMO
Increases of the level of eosinophils in patients with rheumatism depends on the character of the disease course. In patients suffering of latent rheumatism with a subminimal activity, no increased number of circulating eosinophils is observed. It was established that patients with active rheumatism showed an increased subpopulation of eosinophils characterized by low density.
Assuntos
Eosinofilia/diagnóstico , Doenças Reumáticas/diagnóstico , Eosinofilia/sangue , Eosinófilos/fisiologia , Humanos , Contagem de Leucócitos , Recidiva , Doenças Reumáticas/sangueRESUMO
The use of ampicillin, benzylpenicillin, gentamicin, erythromycin, cefamezine or cephaloridine in combination with levamisole aimed at increasing their efficacy and correcting the adverse reactions was studied. Immunological activity of the antibiotics was comparatively characterized by using standard methods. It was shown that the character of the antibiotic effect on the basic factors of nonspecific resistance and immunity was heterogeneous. Schemes for using the antibiotics in combination with levamisole for correcting the detected disorders in the immunity status of the experimental animals were developed.
Assuntos
Antibacterianos/administração & dosagem , Modelos Animais de Doenças , Levamisol/administração & dosagem , Infecções Oportunistas/tratamento farmacológico , Salmonelose Animal/tratamento farmacológico , Salmonella typhimurium/patogenicidade , Adjuvantes Imunológicos , Animais , Quimioterapia Combinada , Tolerância Imunológica/efeitos dos fármacos , Tolerância Imunológica/imunologia , Camundongos , Infecções Oportunistas/etiologia , Infecções Oportunistas/imunologia , Coelhos , Ratos , Salmonelose Animal/etiologia , Salmonelose Animal/imunologia , Salmonella typhimurium/efeitos dos fármacosRESUMO
Some problems connected with the pathogenic action of P. aeruginosa exotoxin under experimental conditions have been studied. The study has revealed that in the process of P. aeruginosa intoxication the development of hypoproteinemia, an increase in the activity of aminotransferases, lactate dehydrogenase and its isoforms occurs in the body. The characteristic features of the process are phasic changes in the activity of ceruloplasmin and in the amount of sulfhydryl groups.
Assuntos
Sangue/efeitos dos fármacos , Exotoxinas/toxicidade , Pseudomonas aeruginosa , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Proteínas Sanguíneas/análise , Ceruloplasmina/análise , Isoenzimas , L-Lactato Desidrogenase/sangue , Coelhos , Ratos , Ratos Endogâmicos , Compostos de Sulfidrila/sangue , Fatores de TempoRESUMO
Some problems of the pathogenic action of P. aeruginosa exotoxin on the body have been experimentally studied. Under the conditions of intoxication produced by P. aeruginosa exotoxin pronounced functional disturbances in the main parameters of central hemodynamics and changes in the coagulation properties of the blood and in the free-radical peroxidation of lipids have been found to occur. The manifestation of pathological changes has been shown to have certain specific features in different organs and to depend on the time elapsed after the introduction of exotoxin into the animals.
Assuntos
Exotoxinas/toxicidade , Pseudomonas aeruginosa , Animais , Sangue/efeitos dos fármacos , Coagulação Sanguínea/efeitos dos fármacos , Cães , Exotoxinas/intoxicação , Hemodinâmica/efeitos dos fármacos , Medições Luminescentes , Masculino , Coelhos , Ratos , Fatores de TempoRESUMO
The virulence and toxigenicity of newly isolated P. aeruginosa strains have been studied in experiments on white mice. These biological properties have been shown to be most pronounced in P. aeruginosa strains isolated from proteins, sometimes greatly exceeding those in strains isolated from healthy persons and the environment. Virulence and the factors which determine it are definitely interrelated in microorganisms and can vary, depending on the conditions of their habitat.
Assuntos
Toxinas Bacterianas/toxicidade , Pseudomonas aeruginosa/patogenicidade , Animais , Humanos , Dose Letal Mediana , Camundongos , Peptídeo Hidrolases/análise , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/enzimologia , Pseudomonas aeruginosa/isolamento & purificação , Ratos , Microbiologia do Solo , Virulência , Microbiologia da ÁguaRESUMO
The results of the electron-microscopic study of P. aeruginosa isolated from patients with surgical inflammatory purulent processes and from the environment (water, soil) are presented. The morphological features of the subcellular structure of virulent and non-virulent P. aeruginosa strains, as well as their adaptation properties, appearing under unfavorable conditions have been established.
Assuntos
Pseudomonas aeruginosa/patogenicidade , Parede Celular/ultraestrutura , Grânulos Citoplasmáticos/ultraestrutura , Humanos , Microscopia Eletrônica , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/ultraestrutura , Microbiologia do Solo , Virulência , Microbiologia da ÁguaRESUMO
The main characteristics of the nonspecific responsiveness of the body have been studied on the model of acute P. aeruginosa intoxication. An increase in phagocytic and lysozyme activity and in the bactericidal properties of blood serum have been shown to occur during the first 6 hours after the introduction of P. aeruginosa exotoxin. The titer of complement and beta-lysin activity decrease. At a later period of observations a sharp drop in all the characteristics under study (except lysozyme activity) occurs.
Assuntos
Exotoxinas/imunologia , Infecções por Pseudomonas/imunologia , Pseudomonas aeruginosa/imunologia , Animais , Atividade Bactericida do Sangue , Proteínas do Sistema Complemento/análise , Imunidade Inata , Lisina/sangue , Fagocitose , CoelhosRESUMO
Experimental study with the use of 131I-albumin has revealed changes in the micro-circulatory blood channel and in the permeability of the blood vessels for albumin under the conditions of pyocyanic intoxication caused partly by purified P. aeruginosa exotoxin.